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Some significant progress was made with just 16 experiments; however, there was the question of curvature and a problem with aliasing.  The next step was to augment the experiment again (see previous post) and leverage the existing data with the next round of experiments.  Based upon the results, there are interactions starting to reveal themselves and our ANOVA results look promising.
ANOVA Results from 2nd Round Augmentation

Source	DF	Sum of Squares	Mean Square	F Ratio
Model	15	244.18958	16.2793	27.1039
Error	8	4.80500	0.6006	Prob > F
C. Total	23	248.99458		<.0001*

Parameter Estimates from 2nd Round Augmentation Fit to Full Factorial Model

Term		Estimate	Std Error	t Ratio	Prob>|t|
Intercept		7.475	0.19375	38.58	<.0001*
Glutamine		-0.875	0.237294	-3.69	0.0062*
EAA		0.2	0.251689	0.79	0.4498
NEAA		0.8	0.345913	2.31	0.0495*
ITS		0.35	0.237294	1.47	0.1785
Lipids		-1.275	0.443937	-2.87	0.0208*
Glutamine*EAA		-0.9375	0.232298	-4.04	0.0038*
Glutamine*NEAA		0.4375	0.232298	1.88	0.0964
Glutamine*ITS		0.2375	0.290625	0.82	0.4375
Glutamine*Lipids		0.4375	0.237294	1.84	0.1025
EAA*NEAA		1.25	0.335585	3.72	0.0058*
EAA*ITS		-0.3875	0.232298	-1.67	0.1338
EAA*Lipids		1.775	0.345913	5.13	0.0009*
NEAA*ITS		-1.175	0.232298	-5.06	0.0010*
NEAA*Lipids		0.6125	0.251689	2.43	0.0410*
ITS*Lipids		0.325	0.237294	1.37	0.2080

Look what happens when the center point values are included:
ANOVA Results from 2nd Round Augmentation with Centerpoints

Source	DF	Sum of Squares	Mean Square	F Ratio
Model	15	245.90930	16.3940	7.0491
Error	11	25.58237	2.3257	Prob > F
C. Total	26	271.49167		0.0012*

Parmeter Estimates from 2nd Round Augmentation fit with Centerpoints

Term		Estimate	Std Error	t Ratio	Prob>|t|
Intercept		7.8526316	0.349862	22.44	<.0001*
Glutamine		-0.875	0.466938	-1.87	0.0877
EAA		-0.03602	0.486128	-0.07	0.9423
NEAA		1.0360197	0.674056	1.54	0.1525
ITS		0.35	0.466938	0.75	0.4692
Lipids		-0.991776	0.866141	-1.15	0.2765
Glutamine*EAA		-0.890296	0.456715	-1.95	0.0772
Glutamine*NEAA		0.3902961	0.456715	0.85	0.4110
Glutamine*ITS		0.1430921	0.570625	0.25	0.8066
Glutamine*Lipids		0.4375	0.466938	0.94	0.3689
EAA*NEAA		1.0611842	0.655992	1.62	0.1340
EAA*ITS		-0.340296	0.456715	-0.75	0.4718
EAA*Lipids		1.5389803	0.674056	2.28	0.0433*
NEAA*ITS		-1.222204	0.456715	-2.68	0.0216*
NEAA*Lipids		0.8485197	0.486128	1.75	0.1087
ITS*Lipids		0.325	0.466938	0.70	0.5009

Confronted with this result, what's the next step in the analysis?  Stay tuned!